COS細胞

COS是衍生自猴腎組織的成纖維細胞樣細胞系，常用於科研用途。

特徵

COS細胞是通過用能產生大T抗原^[1]的猿猴空泡病毒40 （SV40）永生化（immortalizing）CV-1細胞而獲得的^[2] ，而CV-1細胞則來自非洲綠猴的腎臟^[3] 。首字母縮略詞是「COS」，因為COS細胞源自於CV-1細胞，並且攜帶着SV40的遺傳物質^[1] 。COS細胞常用的兩種形式是COS-1細胞和COS-7細胞。COS-1細胞及COS-7細胞都是經帶有編碼野生型T抗原的起始失活突變的SV40轉染得到^[4]^[5]，其中COS-1細胞含有一個來自SV40基因組全部早期區域的組成型拷貝。

科研用途

生物學家在研究SV40時，經常使用COS細胞系。來自這些品系的細胞也經常被轉染，以產生用於分子生物學、生物化學和細胞生物學實驗的重組蛋白^[6] 。當將具有SV40啟動子的表現載體（英语：Expression vector），引入COS細胞時，該載體可以被大T抗原複製。這些COS細胞經過基因修飾，從其自身的基因組中產生T抗原^[6]^[7]。

另見

COS-7細胞
猿猴空泡病毒40

參考資料

^ ^1.0 ^1.1 Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. January 1981, 23 (1): 175–82. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.
^ Jensen FC, Girardi AJ, Gilden RV, Koprowski H. Infection of human and simian tissue cultures with rous sarcoma virus. Proc. Natl. Acad. Sci. U.S.A. July 1964, 52 (1): 53–9. Bibcode:1964PNAS...52...53J. PMC 300571  . PMID 14192657. doi:10.1073/pnas.52.1.53.
^ Fanelli, Alex. COS-7 cell line: Origin and Characteristics. 2016 [28 November 2017]. （原始内容存档于2019-12-22）.
^ Gluzman, Y. SV40-transformed simian cells support the replication of early SV40 mutants.. Cell. 1981-01, 23 (1): 175–82 [2019-12-08]. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.
^ Mellon, P; Parker, V; Gluzman, Y; Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.. Cell. 1981-12, 27 (2 Pt 1): 279–88 [2019-12-08]. PMID 6277501. doi:10.1016/0092-8674(81)90411-6.
^ ^6.0 ^6.1 Aruffo, A. Transient expression of proteins using COS cells. Curr Protoc Mol Biol. November 2002,. Chapter 16: Unit 16.12. ISBN 0471142727. PMID 18265298. doi:10.1002/0471142727.mb1612s60.
^ WEINANDER, Rolf; et al. Heterologous expression of rat liver microsomal glutathione transferase in simian COS cells and Escherichia coli. Biochem. J. 1995, 311 (3): 861–866. PMC 1136080  . PMID 7487942. doi:10.1042/bj3110861.

外部連結

Cellosaurus entry for COS-1（页面存档备份，存于互联网档案馆）
Cellosaurus entry for COS-7（页面存档备份，存于互联网档案馆）

[Gluzman_1981-1] 1.0 ^1.1 Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. January 1981, 23 (1): 175–82. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.

[pmid14192657-2] Jensen FC, Girardi AJ, Gilden RV, Koprowski H. Infection of human and simian tissue cultures with rous sarcoma virus. Proc. Natl. Acad. Sci. U.S.A. July 1964, 52 (1): 53–9. Bibcode:1964PNAS...52...53J. PMC 300571  . PMID 14192657. doi:10.1073/pnas.52.1.53.

[3] Fanelli, Alex. COS-7 cell line: Origin and Characteristics. 2016 [28 November 2017]. （原始内容存档于2019-12-22）.

[a-4] Gluzman, Y. SV40-transformed simian cells support the replication of early SV40 mutants.. Cell. 1981-01, 23 (1): 175–82 [2019-12-08]. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.

[b-5] Mellon, P; Parker, V; Gluzman, Y; Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.. Cell. 1981-12, 27 (2 Pt 1): 279–88 [2019-12-08]. PMID 6277501. doi:10.1016/0092-8674(81)90411-6.

[Aruffo-6] 6.0 ^6.1 Aruffo, A. Transient expression of proteins using COS cells. Curr Protoc Mol Biol. November 2002,. Chapter 16: Unit 16.12. ISBN 0471142727. PMID 18265298. doi:10.1002/0471142727.mb1612s60.

[7] WEINANDER, Rolf; et al. Heterologous expression of rat liver microsomal glutathione transferase in simian COS cells and Escherichia coli. Biochem. J. 1995, 311 (3): 861–866. PMC 1136080  . PMID 7487942. doi:10.1042/bj3110861.

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